Sample output
This is actual output from ProtocolPilot — generated from the Thermo Fisher DNA Extract All Reagents Kit product sheet, 96 fresh blood samples on a Hamilton STAR. This is exactly what a user sees when a protocol is generated.
protocolpilot.com/protocol/new
GENERATED
SAVED
DNA Extract All Reagents Kit – Fresh Blood (EDTA/Citrate/Heparin)
Hamilton STAR · 96 samples · ~18 min
Deck Layout
TRACKS USED
15 / 55
CARRIERS
3
SITES OCCUPIED
7 / 15
FLAGGED
0
Flagged Issues (4)
REAGENTLysis and Stabilizing Solutions require thorough mixing before use
→ Manually mix reagents by inversion 10× before loading to deck
DEAD_VOLUMEReagent reservoir dead volume ~1–2mL per 50mL trough
→ Load 3.5mL Lysis Solution + 3.5mL Stabilizing Solution (96 samples × 20µL + 1.5mL dead volume each)
SPEEDMixing speed critical to avoid foaming in blood samples
→ Mixing set to 100µL/s (moderate) to prevent aerosol/foam formation
OTHERProtocol requires multiple user interventions for sealing/centrifugation
→ Cannot automate without PlateLoc sealer and on-deck centrifuge
Step Sequence (25 steps)
| # | NAME / COMMAND | SOURCE → DEST | VOL | CHANNELS | LLD | SPEED A/D |
|---|---|---|---|---|---|---|
| 1 | Initialize instrument Initialize |
— | — | 11111111 | cLLD-3 | 150/150 |
| 2 | MANUALUser confirms blood samples loaded User Input Confirm 20µL blood samples in all 96 wells |
— | — | 11111111 | cLLD-3 | 150/150 |
| 3 | ↺ LOOP aspirate Lysis Solution to all 96 wells 12× columns | |||||
| 4 | Pick up tips for Lysis Solution transfer 1000ul Channel Tip Pick Up |
tip1/1 | — | 11111111 | cLLD-3 | 150/150 |
| 5 | Aspirate 20µL Lysis Solution Aspirate |
reagent1/1 | 20µL | 11111111 | cLLD-4 | 150/150 |
| 6 | Dispense Lysis Solution to blood samples Dispense |
plt1/1 | 20µL | 11111111 | cLLD-3 | 150/150 |
| 7 | Mix blood with Lysis Solution Dispense 8 mix cycles to homogenize lysate |
plt1/1 | 20µL | 11111111 | cLLD-3 | 100/100 |
| 8 | Eject tips Tip Eject |
waste/1 | — | 11111111 | cLLD-3 | 150/150 |
| 9 | END LOOP | |||||
| 10 | OFF-DECKUser seals plate for centrifugation User Output Remove plate, apply PCR-grade adhesive seal |
— | ||||
| · · · steps 11–24: incubation timer, Stabilizing Solution loop, final centrifugation · · · | ||||||
| 25 | MANUALUser confirms completion User Input Stabilized DNA lysate ready for storage or downstream processing |
— | ||||
Gap Analysis
✓ HAVE
Hamilton STAR with 8-channel LiHa arm
1000µL disposable tips (sufficient for protocol)
96-well flat bottom plates
50mL reagent reservoirs
Standard tip/plate/reagent carriers
✗ NEED
PlateLoc or XPeel sealing device
Automate plate sealing before centrifugation
$15,000–25,000
On-deck centrifuge module
Automate brief centrifugation steps
$30,000–50,000
PCR-grade adhesive plate seals
Required per run — consumable
$200–500 per case (100 seals)
Always validate generated scripts against your Hamilton Venus installation before running.