Early access — now open

Production-ready liquid handling protocols
in minutes, not months.

Paste your assay parameters. ProtocolPilot outputs an optimised Tecan or Hamilton script — with flagged issues, suggested adjustments, and a full gap analysis. No scripting expertise required.

✓  You're on the list. Your £35/month early access rate is reserved.

Locks in £35/month Pro for your first year. No card required now.

Instrument support
Hamilton STAR Available at launch Tecan Fluent Available at launch Tecan EVO Coming soon Beckman Coulter Coming soon
Don't see your instrument? Request it →
The problem

Liquid handling automation has been standard in labs for two decades. The software to program it hasn't kept up. Writing a protocol still requires specialist knowledge most labs don't have — or hours most scientists can't spare. A single failed run wastes reagents, time, and results. That's the problem ProtocolPilot solves.

4–8h
Typical time to write a new protocol from scratch
1 run
Can cost hundreds in reagents when dead volumes are wrong
< 5 min
Average time to generate a validated protocol with ProtocolPilot

From parameters to protocol,
in three steps.

Set up your robot and labware profiles once. After that, every new protocol takes minutes.

STEP 01
🔬

Define your assay

Enter your assay type, sample count, reagent list with volumes, and known constraints. Your robot and labware profiles are saved — you never repeat them.

STEP 02

Dead volumes calculated. Speeds validated. Conflicts flagged.

ProtocolPilot reasons over your instrument profile, labware library, carrier inventory, and reagent properties to build a complete, instrument-aware protocol.

STEP 03
📋

Review, understand, and run

You get six structured outputs — worktable layout, step sequence, flagged issues, gap analysis, and platform scripting notes — with every decision explained.

Sample output

This is real output generated from the Thermo Fisher DNA Extract All Reagents Kit product sheet, run against a Hamilton STAR 4-channel instrument profile. Every carrier position, tip count, aspirate speed, and flag comes directly from the tool — nothing invented.

DNA Extract All — Thermo Fisher · Hamilton STAR 4-Channel · 96 samples
🗂 WORKTABLE LAYOUT
Low-vol tip carrier | Tracks 1–3 | Racks 1–3: 288 × 50µL CO-RE tips (3 full racks)
5×50mL reagent carrier (P/N 187299) | Tracks 4–6 | Pos 1: Lysis Solution (load ≥5mL) · Pos 2: DNA Stabilizing Solution (load ≥5mL)
Standard 5-pos plate carrier | Tracks 7–9 | Pos 1: 96-well PCR plate, samples pre-loaded · Pos 2–5: empty
Waste bin | Track 55 | Fixed waste · CO-RE Gripper stored here — no separate deck position needed
✓ No reach or clearance conflicts — tracks 10–54 unoccupied
🔢 STEP SEQUENCE (excerpt — Phase 1)
Step B — Pick up tips for Lysis Solution dispense
Command: 1000ul Channel Tip Pick Up
Source: Tip carrier Tracks 1–3 · Rack 1 · Positions 1–4 · Channel pattern: 1111
Note: 4 tips retained across all 24 dispense passes — no tip change between passes
Step C — Lysis Solution dispense loop [24 passes × 4 channels = 96 wells]
Command: 1000ul Channel Aspirate → source pos 1 (Lysis Solution) · 20µL/channel · pLLD sensitivity 2
Aspirate speed: 100µL/s · Over-aspirate: 3µL · Transport air: 5µL
Command: 1000ul Channel Dispense → plate pos 1 · 20µL/channel · fixed height · 100µL/s
⚠️ FLAGGED ISSUES
⚠ [ASSUMED] Sample type not confirmed — protocol built for fresh blood/cell culture (2µL, RT incubation). If sample is FTA card, buccal swab, tissue, hair, or FFPE: lysis volume changes to 50–400µL, 95°C incubation required, protocol must be rebuilt
⚠ Each reagent requires only 1,920µL for 96 wells (96×20µL) — but 50mL tubes have ~2mL dead volume. Load ≥5mL per tube to ensure reliable aspiration throughout run
⚠ "Briefly" centrifuge in IFU is not quantified — assumed 30s at 500–1000×g. Operator must confirm speed and duration for their centrifuge before first run
✓ Tip consumption validated — 200 tips total; 3 racks (288 tips) provides adequate headroom
🔍 GAP ANALYSIS
✗ NEED: Low-volume tip carrier (50µL CO-RE tips) · Tracks 1–3 — not in current inventory
✗ NEED: 5×50mL reagent carrier P/N 187299 · Tracks 4–6 — not in current inventory
✗ NEED: Standard 5-position plate carrier · Tracks 7–9 — not in current inventory
Note: No existing deck configuration provided. All carriers are additions. Verify part numbers with Hamilton before ordering.
💾 SCRIPT NOTES (Venus command summary)
Initialize
1000ul Channel Tip Pick Up // Rack 1 pos 1–4 · pattern 1111
LOOP iPass = 0 to 23:
1000ul Channel Aspirate // Trough pos1 · 20µL · pLLD-2 · 100µL/s
1000ul Channel Dispense // Plate pos1 · 20µL · fixed ht · 100µL/s
END LOOP · 1000ul Channel Tip Eject
LOOP iPass = 0 to 23: [Lysis mix — tip change each pass · 5× mix · 18µL · 150µL/s]
User Output // ⏸ PAUSE: operator seals plate, centrifuges, returns, clicks OK
Timer: Start · Timer: Wait for // 180s RT incubation
[Stabilizing Solution dispense + mix — same pattern as lysis phases]
User Output // ⏸ PAUSE: second centrifugation
Runtime estimate: ~39 min (robot active ~29 min · 2 operator pauses ~10 min) · Total tips: ~200 ✓

Six structured outputs,
every time.

Not a wall of text. Structured, actionable sections your team can actually use — and every decision explained.

🗂

Worktable Layout

Every carrier, position, and site assigned. Labware conflicts and reach limitations flagged before you load a tip.

📋

Protocol Summary

A plain-English SOP-style walkthrough with runtime estimate — useful for review, sign-off, and documentation.

🔢

Step Sequence

Numbered steps with source, destination, volume, tip type, aspirate/dispense speeds, and liquid handling technique for every transfer.

⚠️

Flagged Issues

Specific risks with exact values — dead volumes, evaporation windows, carry-over risks — each with a safe alternative.

🔍

Gap Analysis

What you already have vs. what you'd need to buy, with specific carrier names and cost tier for each missing item.

💾

Script Notes

Platform-specific guidance for translating to Fluent Control or Venus — variable naming, TADM settings, and error recovery suggestions.

Start free.
Scale when you're ready.

No credit card required. Early access subscribers get Pro at £35/month for their first year, locked in — then £49/month thereafter. Sign up to lock in the rate.

Free
£0 / forever

For getting started and validating the workflow in your lab.

  • 1 robot profile
  • 3 protocol runs / month
  • All 6 output sections
  • Gap analysis
  • Protocol history
  • Unlimited robot profiles
Built by people who've run the protocols

ProtocolPilot was built by automation engineers with hands-on experience across Tecan, Hamilton, and Beckman Coulter platforms. Every protocol is generated by a system trained on 15+ years of accumulated knowledge about dead volumes, carrier conflicts, reagent incompatibilities, and the things that actually cause runs to fail. This isn't a general-purpose AI wrapper. It's domain expertise in software form.

The protocol is the hardest part.
It shouldn't be.

Join the waitlist and be among the first labs to run ProtocolPilot at launch.

✓  You're on the list. Your £35/month early access rate is reserved.

Locks in £35/month Pro for your first year. No card required now.