Dead Volume
Calculating and minimising dead volume in liquid handling protocols across labware and instrument types.
All
Tecan
Hamilton
Hamilton STAR
Protocol Scripting
Protocol Optimisation
Dead Volume
QA & Validation
Venus Scripting
2026-06-24
Dead Volume in Hamilton Reagent Reservoirs: What You're Leaving Behind and Why It Matters
Dead volume in flat-bottom Hamilton reservoirs is geometry-driven and calculable — here's how to get the number right and avoid run failures.
2026-06-24Dead Volume in Microplates: Geometry, Accuracy, and Why Your Wells Are Never Quite Empty
Dead volume in microplates is a per-well, per-transfer problem at the 5-500 µL scale — here's how plate format and tip selection change the calculation.
2026-06-23Dead Volume in Tecan Reagent Troughs: Why the Taper Changes Everything
Tecan's tapered troughs concentrate liquid for better low-volume aspiration, but the non-linear geometry means the flat-bottom dead volume formula doesn't apply.
2026-06-22Hamilton vs Tecan Troughs: A Dead Volume Comparison
Hamilton flat-bottom and Tecan tapered troughs make different geometric trade-offs that produce meaningfully different dead volume profiles — here's how to choose and migrate between them.